Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602, Athens, GA, USA.
Plant Cell Rep. 1992 Mar;11(2):71-5. doi: 10.1007/BF00235256.
Somatic embryos were induced on peanut (Arachis hypogaea) leaflets from aseptically germinated embryo axes. Leaflet size influenced percent somatic embryogenesis; 5-8 mm long cut leaflets were superior to 2-3 mm long uncut leaflets. Maximum embryogenesis of 14.6% was obtained after a 15 d incubation on induction medium (modified MS with B5 vitamins, 30 g/l sucrose, 4 g/l Gel-Gro, 40 mg/l 2,4-D +0.2 mg/l kinetin) followed by transfer to a secondary medium with 5 mg/l 2,4-D+0.2 mg/l kinetin. Primary somatic embryos were fused along the axes with no distinct cotyledons, but secondary embryos had single axes with two cotyledons. Other treatments had lower percent embryogenesis, no secondary embryogenesis, and embryos with single axes with two cotyledons. Some somatic embryos converted into normal plants capable of greenhouse survival.
体细胞胚从无菌萌发的胚胎轴上的花生（Arachis hypogaea）小叶中诱导产生。小叶大小影响体细胞胚发生的百分比；5-8 毫米长的切段小叶优于 2-3 毫米长的未切段小叶。在诱导培养基（改良 MS 加 B5 维生素、30 g/l 蔗糖、4 g/l Gel-Gro、40 mg/l 2,4-D+0.2 mg/l 激动素）上培养 15 天后，获得了最高的 14.6%的胚胎发生，然后转移到含有 5 mg/l 2,4-D+0.2 mg/l 激动素的二级培养基上。初级体细胞胚沿着没有明显子叶的轴融合，但次级胚具有单个轴和两个子叶。其他处理的胚胎发生百分比较低，没有次级胚胎发生，且胚胎具有单个轴和两个子叶。一些体细胞胚转化为能够在温室中存活的正常植物。
