Tissue Culture for Crops Project, Department of Botany, Colorado State University, 80523, Fort Collins, CO, USA.
Plant Cell Rep. 1986 Dec;5(6):452-6. doi: 10.1007/BF00269640.
During induction of somatic embryogenesis from immature embryos of soybean, a smooth-shiny and a rough callus were obtained. The smooth-shiny type developed from callus derived from cotyledonary tissue and cultured on media containing 10 mg/l 2,4-D. The rough type was derived from immature embryos, cotyledons, hypocotyls and hypocotyl segments from germinated seedlings on a medium containing various growth regulators. Plants were obtained from the smooth-shiny type but rough callus did not differentiate into plants. The conditions for formation of both callus types and regeneration of mature soybean plants from the smooth-shiny type were defined.
在大豆未成熟胚体胚发生的诱导过程中，得到了光滑闪亮型和粗糙型愈伤组织。光滑闪亮型是由子叶组织来源的愈伤组织在含有 10mg/L 2,4-D 的培养基上培养发育而来。粗糙型则来自于未成熟的胚、子叶、下胚轴和萌发幼苗的下胚轴切段，在含有各种生长调节剂的培养基上培养。植株是从光滑闪亮型愈伤组织中获得的，但粗糙型愈伤组织未能分化为植株。确定了两种愈伤组织类型的形成条件以及从光滑闪亮型再生成熟大豆植株的条件。
