Durand-Tardif M, Broglie R, Slightom J, Tepfer D
J Mol Biol. 1985 Dec 5;186(3):557-64. doi: 10.1016/0022-2836(85)90130-5.
The incorporation of transferred DNA (T-DNA) from the Ri plasmid of Agrobacterium rhizogenes into the chromosomal DNA of higher plants is correlated with the appearance of a complex phenotype. The transformed genotype and phenotype undergo Mendelian inheritance. Through studies of Ri T-DNA content and transcription in Nicotiana tabacum, we have delineated a particular part of this foreign DNA as the likely source of the transformed phenotype. One inducible/repressible aspect of the transformed phenotype is termed T' and is correlated with the presence of a supplementary Ri T-DNA-encoded transcript. This transcript is found specifically in leaves, whereas most of the other T-DNA transcripts are more abundant in roots. The T' phenotype does not appear to be due to structural changes in the Ri T-DNA. It is inherited in a dominant Mendelian fashion. We propose that the T' phenotype is caused by heritable changes in the regulation of Ri T-DNA expression. We comment on the potential of this system as a model for studying eukaryotic gene expression.
发根农杆菌Ri质粒的转移DNA（T-DNA）整合到高等植物的染色体DNA中与复杂表型的出现相关。转化后的基因型和表型遵循孟德尔遗传规律。通过对烟草中Ri T-DNA含量和转录的研究，我们已经确定了这种外源DNA的一个特定部分可能是转化表型的来源。转化表型的一个可诱导/可抑制方面被称为T'，它与一个额外的由Ri T-DNA编码的转录本的存在相关。这个转录本只在叶片中特异性地发现，而大多数其他T-DNA转录本在根中更为丰富。T'表型似乎不是由Ri T-DNA的结构变化引起的。它以显性孟德尔方式遗传。我们提出T'表型是由Ri T-DNA表达调控中的可遗传变化引起的。我们评论了这个系统作为研究真核基因表达模型的潜力。
