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来自根癌农杆菌的pTi-15955的T-DNA在向日葵冠瘿瘤中被转录成至少七种多聚腺苷酸化RNA。

T-DNA of pTi-15955 from Agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated RNAs in a sunflower crown gall tumor.

作者信息

Murai N, Kemp J D

出版信息

Nucleic Acids Res. 1982 Mar 11;10(5):1679-89. doi: 10.1093/nar/10.5.1679.

DOI:10.1093/nar/10.5.1679
PMID:6175956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320558/
Abstract

Northern blot hybridization analysis of polysomal polyadenylated RNA isolated from sunflower crown gall tumor PSCG-15955 demonstrated that a minimum of seven RNAs were transcribed from T-DNA of pTi-15955 from Agrobacterium tumefaciens. The sizes of the T-DNA transcripts were 1.8, 1.6, 1.5, 1.1, 1.0 kilo bases (kb) and two transcripts of 0.8 kb long. The relative abundance of these polyadenylated RNAs varied greatly, the 1.0 kb RNA being the most abundant and the 1.6 kb RNA being the least abundant. Assignment of map locations of the seven polyadenylated RNAs indicated that the conserved region of T-DNA which may play a central role in tumorigenesis contained four RNAs of 1.8, 1.1, 0.8(a) and a portion of 0.8(b) kb long.

摘要

对从向日葵冠瘿瘤PSCG - 15955中分离出的多聚核糖体聚腺苷酸化RNA进行的Northern印迹杂交分析表明，至少有7种RNA是由根癌农杆菌的pTi - 15955的T - DNA转录而来的。T - DNA转录本的大小分别为1.8、1.6、1.5、1.1、1.0千碱基（kb）以及两个0.8 kb长的转录本。这些聚腺苷酸化RNA的相对丰度差异很大，1.0 kb的RNA最为丰富，而1.6 kb的RNA最不丰富。对这7种聚腺苷酸化RNA的图谱位置进行定位表明，T - DNA的保守区域可能在肿瘤发生中起核心作用，该区域包含4种RNA，长度分别为1.8、1.1、0.8(a)以及0.8(b) kb的一部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b92/320558/f99e958cddd1/nar00374-0259-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b92/320558/f99e958cddd1/nar00374-0259-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b92/320558/f99e958cddd1/nar00374-0259-a.jpg

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1
T-DNA of pTi-15955 from Agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated RNAs in a sunflower crown gall tumor.来自根癌农杆菌的pTi-15955的T-DNA在向日葵冠瘿瘤中被转录成至少七种多聚腺苷酸化RNA。
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引用本文的文献

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Map locations of five transcripts homologous to TR-DNA in tobacco and sunflower crown gall tumors.烟草和向日葵冠瘿瘤中与TR-DNA同源的五个转录本的图谱定位。
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Deletion analysis of the mannopine synthase gene promoter in sunflower crown gall tumors and Agrobacterium tumefaciens.向日葵冠瘿瘤和根癌土壤杆菌中甘露碱合成酶基因启动子的缺失分析
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