Cho Hyeyoung, Ra Chae-Hun, Kim Sung-Koo
Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea.
J Microbiol Biotechnol. 2014 Feb 28;24(2):264-9. doi: 10.4014/jmb.1307.07054.
For the production of ethanol from seaweed as the source material, thermal acid hydrolysis and enzymatic saccharification were carried out for monosugars production of 25.5 g/l galactose and 7.6 g/l glucose using Gelidium amansii. The fermentation was performed with Pichia stipitis KCTC 7228 or Saccharomyces cerevisiae KCCM 1129. When wild P. stipitis and S. cerevisiae were used, the ethanol productions of 11.2 g/l and 6.9 g/l were produced, respectively. The ethanol productions of 16.6 g/l and 14.6 g/l were produced using P. stipitis and S. cerevisiae acclimated to high concentration of galactose, respectively. The yields of ethanol fermentation increased to 0.5 and 0.44 from 0.34 and 0.21 using acclimated P. stipitis and S. cerevisiae, respectively. Therefore, acclimation of yeasts to a specific sugar such as galactose reduced the glucose-induced repression on the transport of galactose.
以海藻为原料生产乙醇时，使用石花菜进行热酸水解和酶促糖化，以生产单糖，其中半乳糖含量为25.5克/升，葡萄糖含量为7.6克/升。使用树干毕赤酵母KCTC 7228或酿酒酵母KCCM 1129进行发酵。当使用野生树干毕赤酵母和酿酒酵母时，乙醇产量分别为11.2克/升和6.9克/升。使用适应高浓度半乳糖的树干毕赤酵母和酿酒酵母时，乙醇产量分别为16.6克/升和14.6克/升。使用适应后的树干毕赤酵母和酿酒酵母进行乙醇发酵时，乙醇产量分别从0.34和0.21提高到0.5和0.44。因此，使酵母适应特定糖类（如半乳糖）可减少葡萄糖对半乳糖转运的抑制作用。
