Sunwoo In Yung, Kwon Jung Eun, Nguyen Trung Hau, Ra Chae Hun, Jeong Gwi-Taek, Kim Sung-Koo
Department of Biotechnology, Pukyong National University, Busan, 48513, South Korea.
Appl Biochem Biotechnol. 2017 Nov;183(3):966-979. doi: 10.1007/s12010-017-2476-6. Epub 2017 Apr 29.
Conditions for ethanol production were evaluated using waste seaweed obtained from Gwangalli beach, Busan, Korea, after strong winds on January 15, 2015. Eleven types of seaweed were identified, and the proportions of red, brown, and green seaweed wastes were 26, 46, and 28%, respectively. Optimal pretreatment conditions were determined as 8% slurry content, 286 mM HSO for 90 min at 121 °C. Enzymatic saccharification with 16 units/mL Celluclast 1.5L and Viscozyme L mixture at 45 °C for 48 h was carried out as optimal condition. A maximum monosaccharide concentration of 30.2 g/L was obtained and used to produce ethanol. Fermentation was performed with single or mixed yeasts of non-adapted and adapted Saccharomyces cerevisiae KCTC 1126 and Pichia angophorae KCTC 17574 to galactose and mannitol, respectively. The maximum ethanol concentration and yield of 13.5 g/L and Y of 0.45 were obtained using co-culture of adapted S. cerevisiae and P. angophorae.
利用2015年1月15日大风过后从韩国釜山广安里海滩获取的废弃海藻，对乙醇生产条件进行了评估。鉴定出11种海藻，其中红藻、褐藻和绿藻废弃物的比例分别为26%、46%和28%。确定最佳预处理条件为浆料含量8%，在121℃下用286 mM硫酸处理90分钟。以16单位/毫升的纤维素酶1.5L和里氏木霉混合酶在45℃下进行48小时的酶促糖化作为最佳条件。获得了最高30.2克/升的单糖浓度并用于生产乙醇。分别用未适应和适应半乳糖和甘露醇的酿酒酵母KCTC 1126和安哥拉毕赤酵母KCTC 17574的单一酵母或混合酵母进行发酵。使用适应的酿酒酵母和安哥拉毕赤酵母共培养，获得了最高乙醇浓度13.5克/升和产率Y为0.45。
