Sunwoo In Yung, Nguyen Trung Hau, Sukwong Pailin, Jeong Gwi-Teak, Kim Sung-Koo
Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
J Microbiol Biotechnol. 2018 Mar 28;28(3):401-408. doi: 10.4014/jmb.1708.08041.
The waste seaweed from Gwangalli beach, Busan, Korea was utilized as biomass for ethanol production. (brown seaweed, Mojaban in Korean name) comprised 72% of the biomass. The optimal hyper thermal acid hydrolysis conditions were obtained as 8% slurry contents, 138 mM sulfuric acid, and 160°C of treatment temperature for 10 min with a low content of inhibitory compounds. To obtain more monosaccharides, enzymatic saccharification was carried out with Viscozyme L for 48 h. After pretreatment, 34 g/l of monosaccharides were obtained. and were selected as optimal co-fermentation yeasts to convert all of the monosaccharides in the hydrolysate to ethanol. Co-fermentation was carried out with various inoculum ratios of and . The maximum ethanol concentration of 16.0 g/l was produced using and in a 3:1 inoculum ratio, with an ethanol yield of 0.47 in 72 h. Ethanol fermentation using yeast co-culture may offer an efficient disposal method for waste seaweed while enhancing the utilization of monosaccharides and production of ethanol.
韩国釜山广安里海滩的废弃海藻被用作生产乙醇的生物质原料。（褐藻，韩语名为Mojaban）占生物质的72%。最佳超高温酸水解条件为：浆料含量8%、硫酸浓度138 mM、处理温度160°C、处理时间10分钟，且抑制性化合物含量较低。为获得更多单糖，用嗜热栖热放线菌淀粉酶进行48小时的酶糖化。预处理后，获得了34 g/l的单糖。选择 和 作为最佳共发酵酵母，将水解产物中的所有单糖转化为乙醇。以不同接种比例对 和 进行共发酵。以3:1的接种比例使用 和 时，乙醇最高浓度达到16.0 g/l，72小时内乙醇产率为0.47。使用酵母共培养进行乙醇发酵，可为废弃海藻提供一种有效的处理方法，同时提高单糖利用率和乙醇产量。
