Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University, Anseong 17579, Republic of Korea.
Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea.
J Microbiol Biotechnol. 2020 Dec 28;30(12):1912-1918. doi: 10.4014/jmb.2007.07037.
Hyper-thermal (HT) acid hydrolysis of red seaweed was performed using 12% (w/v) slurry and an acid mix concentration of 180 mM at 150°C for 10 min. Enzymatic saccharification when using a combination of Celluclast 1.5 L and CTec2 at a dose of 16 U/ml led to the production of 12.0 g/l of reducing sugar with an efficiency of enzymatic saccharification of 13.2%. After the enzymatic saccharification, 2,3-butanediol (2,3-BD) fermentation was carried out using an engineered strain. The use of HT acid-hydrolyzed medium with 1.9 g/l of 5-hydroxymethylfurfural showed a reduction in the lag time from 48 to 24 h. The 2,3-BD concentration and yield coefficient at 72 h were 14.8 g/l and 0.30, respectively. Therefore, HT acid hydrolysis and the use of the engineered strain can enhance the overall 2,3-BD yields from seaweed.
采用 12%（w/v）的料浆和 180mM 的酸混合浓度，在 150°C 下对红海藻类进行超热（HT）酸水解 10 分钟。使用纤维素酶 1.5 L 和 CTec2 的组合，剂量为 16U/ml，进行酶解，可产生 12.0g/L 的还原糖，酶解效率为 13.2%。酶解后，采用工程化菌株进行 2,3-丁二醇（2,3-BD）发酵。使用 HT 酸水解培养基，其中含有 1.9g/L 的 5-羟甲基糠醛，可将迟滞时间从 48 小时缩短至 24 小时。72 小时时，2,3-BD 的浓度和产率系数分别为 14.8g/L 和 0.30。因此，HT 酸水解和工程化菌株的使用可以提高海藻中 2,3-BD 的总产率。
