Department of Life Science, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.
Plant Cell Rep. 1993 Nov;13(1):49-53. doi: 10.1007/BF00232315.
The effects have been studied of the non-ionic surfactants, Plutonic F-68, Tween 20 or Triton X-100, on shoot regeneration from cultured jute (Corchorus capsularis L.) cotyledons with attached petioles. This group of non-ionic surfactants was selected in order to determine a possible relationship between the physico-chemical properties of individual compounds and their observable effects on plant morphogenesis in cultured jute cotyledons. Supplementation of culture medium with 0.001-0.5% (w/v) Pluronic F-68 increased the mean percentage of cotyledons producing shoots and the mean number of shoots/cotyledon, with maximal responses at 0.5% (w/v). By contrast, Tween 20 produced maximal effects at 0.001% (v/v), with inhibition of shoot formation at 0.5% (v/v). In both cases, phenotypically normal plants were recovered which could be grown to maturity. Culture of cotyledons with 0.001% (v/v) Triton X-100 similarly increased both the percentage of cotyledons producing shoots and the number of shoots/cotyledon. However, these shoots did not develop into mature plants. Additionally, shoots did not regenerate from cotyledons cultured with Triton at 0.01-0.5% (v/v). These results demonstrate mat there is an apparent relationship between the hydrophilic-hydrophobic (HLB) balance of surfactants which determine their cell permeabilising properties and consequently, their effects on morphogenesis.
研究了非离子表面活性剂 Pluronic F-68、Tween 20 或 Triton X-100 对带叶柄培养的黄麻（Corchorus capsularis L.）子叶再生的影响。选择这组非离子表面活性剂是为了确定单个化合物的物理化学性质与其在培养的黄麻子叶中对植物形态发生的观察到的影响之间可能存在的关系。在培养基中添加 0.001-0.5%（w/v）Pluronic F-68 可提高产生芽的子叶的平均百分比和每个子叶的平均芽数，在 0.5%（w/v）时达到最大响应。相比之下，Tween 20 在 0.001%（v/v）时产生最大效应，在 0.5%（v/v）时抑制芽形成。在这两种情况下，都回收了表型正常的植物，可以生长成熟。用 0.001%（v/v）Triton X-100 培养子叶同样提高了产生芽的子叶的百分比和每个子叶的芽数。然而，这些芽不能发育成成熟的植物。此外，用 Triton 在 0.01-0.5%（v/v）培养子叶时，芽不会再生。这些结果表明，表面活性剂的亲水-亲油（HLB）平衡与其细胞通透性质有关，从而与其对形态发生的影响有关。
