Murhammer D W, Goochee C F
Department of Chemical Engineering, University of Houston, Texas 77204-4792.
Biotechnol Prog. 1990 Mar-Apr;6(2):142-8. doi: 10.1021/bp00002a008.
The nonionic surfactant Pluronic F-68 polyol is commonly used to protect cultured animal cells from the detrimental effects of sparging. In this study we investigated the structural features of the Pluronic F-68 molecule responsible for this protective behavior. Poly(oxyethylene)-poly(oxypropylene) block copolymer polyols of various molecular weights and percentages of hydrophobe (poly(oxypropylene], including both Pluronic and reverse Pluronic polyols, were considered. The potential toxicity of these agents was examined in the absence of sparging (i.e., in spinner flasks) by using the attachment-independent Sf9 insect cell line as a model system. Each polyol resulted in one of three distinct types of behavior in these spinner flask experiments: (1) cells lysed at an exponential rate, (2) inhibition of cell growth (i.e., no net cell growth), or (3) uninhibited cell growth. It was then shown that all of the Pluronic and reverse Pluronic polyols that did not inhibit cell growth provided protection from sparging in the bioreactors used in this study; thus, finding a polyol that protected cells was synonymous with finding one that did not inhibit cell growth. The ability of these polyols to protect animal cells in sparged bioreactors was found to correlate well with the hydrophilic-lipophilic balance (HLB). Those polyols with the largest HLB values were found to be protective agents. These poly(oxyethylene)-poly(oxypropylene) polyols were also shown to be more effective protective agents than pure poly(oxyethylene); thus, the presence of the hydrophobe (poly(oxypropylene] is important in their ability to serve as protective agents.
非离子表面活性剂普朗尼克F - 68多元醇常用于保护培养的动物细胞免受鼓泡的有害影响。在本研究中，我们研究了普朗尼克F - 68分子中负责这种保护行为的结构特征。考虑了各种分子量和疏水基（聚氧化丙烯）百分比的聚（氧化乙烯） - 聚（氧化丙烯）嵌段共聚物多元醇，包括普朗尼克和反普朗尼克多元醇。通过使用不依赖贴壁的Sf9昆虫细胞系作为模型系统，在无鼓泡（即在旋转瓶中）的情况下检测了这些试剂的潜在毒性。在这些旋转瓶实验中，每种多元醇都导致了三种不同类型的行为之一：（1）细胞以指数速率裂解，（2）细胞生长受到抑制（即无净细胞生长），或（3）细胞生长未受抑制。然后表明，所有不抑制细胞生长的普朗尼克和反普朗尼克多元醇在本研究使用的生物反应器中都能提供免受鼓泡的保护；因此，找到一种能保护细胞的多元醇就等同于找到一种不抑制细胞生长的多元醇。发现这些多元醇在鼓泡生物反应器中保护动物细胞的能力与亲水 - 亲油平衡（HLB）密切相关。发现那些HLB值最大的多元醇是保护剂。这些聚（氧化乙烯） - 聚（氧化丙烯）多元醇也被证明比纯聚（氧化乙烯）更有效地作为保护剂；因此，疏水基（聚氧化丙烯）的存在对于它们作为保护剂的能力很重要。
