Otsu Hydrobiological Station, Kyoto University, Shimosakamoto, 520-01, Otsu, Japan.
Microb Ecol. 1990 May;19(3):227-38. doi: 10.1007/BF02017167.
The effect of carbon∶nitrogen∶phosphorus (C∶N∶P) ratio of organic substrates on the regeneration of ammonium and phosphate was investigated by growing natural assemblages of freshwater bacteria in mineral media supplemented with the simple organic C, N, and P sources (glucose, asparagine, and sodium glycerophosphate, respectively) to give 25 different substrate C∶N∶P ratios. Both ammonium and phosphate were regenerated when C∶N and N∶P atomic ratios of organic substrates were ≤10∶1 and ≤16∶1, respectively. Only ammonium was regenerated when C∶N and N∶P ratios were ≤10∶1 and ≥10-20∶1, respectively. On the other hand, neither ammonium nor phosphate was regenerated when C∶N and N∶P ratios were ≥15∶1 and ≥5∶1, respectively. In no case was phosphate alone regenerated. As bacteria were able to alter widely the C∶N∶P ratio of their biomass, the growth yield of bacteria appeared primarily dependent on the substrate carbon concentration, irrespective of a wide variation in the substrate C∶N∶P ratio.
通过在含有简单有机 C、N 和 P 源（分别为葡萄糖、天冬酰胺和甘油磷酸钠）的矿物培养基中培养淡水细菌的自然组合，研究了有机基质的碳氮磷（C:N:P）比例对铵和磷酸盐再生的影响，以得到 25 种不同的基质 C:N:P 比例。当有机基质的 C:N 和 N:P 原子比分别≤10:1 和≤16:1 时，铵和磷酸盐都能被再生。当 C:N 和 N:P 比值分别≤10:1 和≥10-20:1 时，仅铵被再生。另一方面，当 C:N 和 N:P 比值分别≥15:1 和≥5:1 时，铵和磷酸盐都不能被再生。在任何情况下，磷酸盐都不能单独被再生。由于细菌能够广泛改变其生物量的 C:N:P 比，因此细菌的生长产量主要取决于基质中的碳浓度，而与基质 C:N:P 比的广泛变化无关。
