VTT, Biotechnical Laboratory, PL 202, SF-02151, Espoo, Finland.
Plant Cell Rep. 1993 May;12(7-8):435-40. doi: 10.1007/BF00234708.
Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic Geneticin(R). Enzymatic activity was tested in the Geneticin(R) resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.
大麦（Hordeum vulgare L. cv Pokko）悬浮培养细胞分别用两种含有新霉素磷酸转移酶和β-葡萄糖醛酸酶基因的质粒稳定转化。转化培养物用抗生素 Geneticin（R）选择。在 Geneticin（R）抗性培养物中检测到酶活性，其中 96%可检测到新霉素磷酸转移酶。非选择标记物，以β-葡萄糖醛酸酶活性检测，在 40%的抗性培养物中表达。Southern 印迹杂交证实了稳定转化。
