Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.
Plant Cell Rep. 1984 Jun;3(3):95-7. doi: 10.1007/BF02441008.
Callus and suspension cell lines were derived from haploid barley embryos produced by the Bulbosum method. Embryos 1 to 2 mm long callused on medium containing a low concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Fast-growing nodular, beige callus (Type 1), slow-growing, light brown, watery callus (Type 2) and a dense, light yellow, nodular callus (Type 3) were recovered. Type 3 callus was embryogenic and was produced on embryos 1 to 2 mm in length. Although callus cultures gradually became polyploid, a small proportion of haploid cells was retained and the majority of regenerated plantlets were haploid. The organogenic potential of long-term (Type 1) callus cultures was generally low and decreased with time. Attempts to inducede novo shoot formation in Type 1 cultures were not successful.
愈伤组织和悬浮细胞系是从小麦单倍体胚中诱导而来的，这些胚是通过 Bulbosum 方法产生的。长度为 1 到 2 毫米的胚在含有低浓度 2,4-二氯苯氧乙酸（2,4-D）的培养基上形成愈伤组织。快速生长的结节状、米色愈伤组织（1 型）、生长缓慢、浅棕色、水样的愈伤组织（2 型）和致密、浅黄色、结节状的愈伤组织（3 型）被回收。3 型愈伤组织是胚胎发生的，是由 1 到 2 毫米长的胚产生的。虽然愈伤组织培养物逐渐成为多倍体，但保留了一小部分单倍体细胞，并且大多数再生的植物是单倍体。长期（1 型）愈伤组织培养物的器官发生潜力通常较低，并且随着时间的推移而降低。在 1 型培养物中诱导新的芽形成的尝试没有成功。
