Weil-Hillman G, Zakay-Rones Z
Scan Electron Microsc. 1985(Pt 4):1679-86.
We investigated the destructive capability of three influenza A strains, Victoria, PR8, and their recombinant X47, against the human lymphoma cell line Daudi. Both Victoria and X47 strains share the same envelope glycoproteins (H3N2), while PR8, the second parental strain of X47, differs in its envelope glycoproteins (HON1). The H3N2 strains and particularly the X47 recombinant were cytotoxic to Daudi cells while the HON1 strain was not. To reduce the virulence of the oncolytic viruses, we inactivated them either with heat (56 degrees C/45 min) or with formalin. Both treatments significantly reduced the infectivity of the viruses. The X47 virus treated with formalin retained its hemagglutinin (HA) and neuraminidase (NA) activities but lost its cytotoxic potential. On the other hand, the heat-treated X47 virus retained its HA activity, lost its NA activity, but preserved its cytotoxic potential. Thus, the heat-inactivated X47 virus (X56) was the most effective non-virulent oncolytic agent we tested. The X56 virus arrested Daudi cell multiplication in-vitro by inhibiting cellular DNA synthesis. The mechanism by which X56 inhibited Daudi cell DNA synthesis was not related to interferon induction in Daudi cells, and did not necessarily involve the activation of the Epstein-Barr virus (EBV) genome present in Daudi cells. Although the mechanism remains unclear, the oncolytic potential of the X56 virus on Daudi cells was demonstrated.
我们研究了三种甲型流感病毒株，即维多利亚株、PR8株及其重组体X47，对人淋巴瘤细胞系Daudi的破坏能力。维多利亚株和X47株具有相同的包膜糖蛋白（H3N2），而X47的第二个亲本株PR8，其包膜糖蛋白（HON1）则不同。H3N2株，特别是X47重组体，对Daudi细胞具有细胞毒性，而HON1株则没有。为了降低溶瘤病毒的毒力，我们用热（56℃/45分钟）或福尔马林将它们灭活。两种处理都显著降低了病毒的感染性。用福尔马林处理的X47病毒保留了其血凝素（HA）和神经氨酸酶（NA）活性，但失去了细胞毒性潜力。另一方面，热处理的X47病毒保留了其HA活性，失去了NA活性，但保留了其细胞毒性潜力。因此，热灭活的X47病毒（X56）是我们测试的最有效的无毒溶瘤剂。X56病毒通过抑制细胞DNA合成在体外阻止了Daudi细胞的增殖。X56抑制Daudi细胞DNA合成的机制与Daudi细胞中干扰素的诱导无关，也不一定涉及Daudi细胞中存在的爱泼斯坦-巴尔病毒（EBV）基因组的激活。虽然机制尚不清楚，但X56病毒对Daudi细胞的溶瘤潜力得到了证实。
