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转录组分析以鉴定甘薯潜在的花特异性基因和开花调控相关基因。

Transcriptome analysis to identify putative floral-specific genes and flowering regulatory-related genes of sweet potato.

作者信息

Tao Xiang, Gu Ying-Hong, Jiang Yu-Song, Zhang Yi-Zheng, Wang Hai-Yan

机构信息

College of Life Sciences, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Sichuan University.

出版信息

Biosci Biotechnol Biochem. 2013;77(11):2169-74. doi: 10.1271/bbb.130218. Epub 2013 Nov 7.

DOI:10.1271/bbb.130218
PMID:24200775
Abstract

Sweet potato flowers were collected for a transcriptome analysis to identify the putative floral-specific and flowering regulatory-related genes by using the RNA-sequencing technique. Pair-end short reads were de novo assembled by an integrated strategy, and then the floral transcriptome was carefully compared with several published vegetative transcriptomes. A total of 2595 putative floral-specific and 2928 putative vegetative-specific transcripts were detected. We also identified a large number of transcripts similar to the key genes in the flowering regulation network of Arabidopsis thaliana.

摘要

采集甘薯花用于转录组分析，通过RNA测序技术鉴定假定的花特异性和开花调控相关基因。采用综合策略对双末端短读段进行从头组装，然后将花转录组与几个已发表的营养器官转录组进行仔细比较。共检测到2595个假定的花特异性转录本和2928个假定的营养器官特异性转录本。我们还鉴定出大量与拟南芥开花调控网络中的关键基因相似的转录本。

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