College of Life Sciences, Sichuan Normal University, Chengdu, Sichuan 610101, China.
College of Life Science & Forestry, Chongqing University of Art & Science, Yongchuan, Chongqing 402160, China.
Biomed Res Int. 2017;2017:6847532. doi: 10.1155/2017/6847532. Epub 2017 May 30.
As an indicator of the antioxidant capability of plants, catalase can detoxify reactive oxygen species (ROS) generated by environmental stresses. Sweet potato is one of the top six most important crops in the world. However, its catalases remain largely unknown. In this study, a catalase encoding gene, (accession number: KY615708), was identified and cloned from sweet potato cv. Xushu 18. It contained a 1479 nucleotides' open reading frame (ORF). S-R-L, Q-K-L, and a putative calmodulin binding domain were located at the C-terminus of IbCAT2, which suggests that IbCAT2 could be a peroxisomal catalase. Next-generation sequencing (NGS) based quantitative analyses showed that was mainly expressed in young leaves and expanding tuberous roots under normal conditions. When exposed to 10% PEG6000 or 200 mmol/L NaCl solutions, was upregulated rapidly in the first 11 days and then downregulated, although different tissues showed different degree of change. Overexpression of conferred salt and drought tolerance in and . The positive response of to abiotic stresses suggested that might play an important role in stress responses.
作为植物抗氧化能力的一个指标，过氧化氢酶可以解毒环境胁迫产生的活性氧（ROS）。番薯是世界上最重要的六大作物之一。然而，其过氧化氢酶的情况在很大程度上仍是未知的。在这项研究中，从番薯品种徐薯 18 中鉴定并克隆了一个过氧化氢酶编码基因，（登录号：KY615708）。它包含一个 1479 个核苷酸的开放阅读框（ORF）。IbCAT2 的 C 末端有 S-R-L、Q-K-L 和一个假定的钙调蛋白结合域，这表明 IbCAT2 可能是过氧化物酶体过氧化氢酶。基于下一代测序（NGS）的定量分析表明，在正常条件下，主要在幼叶和扩展的块根中表达。当用 10%PEG6000 或 200mmol/L NaCl 溶液处理时，在最初的 11 天内迅速上调，然后下调，尽管不同组织表现出不同程度的变化。在 和 中过表达 赋予了耐盐和耐旱性。对非生物胁迫的积极响应表明 可能在胁迫响应中发挥重要作用。
