Key Laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen 518057, PR China; Department of Biology and Chemistry, and the State Key Laboratory of Marine Pollution, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
Biosens Bioelectron. 2014 Mar 15;53:406-13. doi: 10.1016/j.bios.2013.09.068. Epub 2013 Oct 9.
A piezoelectric biosensor for detection of endocrine disrupting chemicals (EDCs) was developed by incorporating chemical/biochemical recognition elements on the ceramic resonator surface for competitive binding assays. A facile electrodeposition was employed to modify the sensor surface with Au nanoparticles, which increased the surface area and enhanced the binding capacity of the immobilized probes. Thiol-labeled long chain hydrocarbon with bisphenol A (BPA) as head group was synthesized and self-assembled on the Au nanoparticle surface as the sensing probes, which showed a linear response upon the binding of estrogen receptor (ER-α) ranging from 1 to 30 nM. Detection of estrone, 17β-estradiol and BPA was achieved by integrating a competitive binding assay with the piezoelectric sensor. In this detection scheme, different concentrations of EDCs were incubated with 30 nM of ER-α, and the un-bounded ER-α in the solution was captured by the probes immobilized on the ceramic resonator, which resulted in the frequency changes for different EDCs. The biosensor assay exhibited a linear response to EDCs with a low detection limit of 2.4-2.9 nM (S/N=3), and required only a small volume of sample (1.5 µl) with the assay time of 2h. The performance of the biosensor assay was also evaluated for rapid and facile determination of EDCs of environmental relevant concentrations in drinking water and seawater, and the recovery rate was in the range between 94.7% and 109.8%.
一种用于检测内分泌干扰化学物质（EDCs）的压电生物传感器是通过在陶瓷谐振器表面上结合化学/生物化学识别元件来开发的，用于竞争性结合分析。采用简便的电沉积方法在传感器表面修饰金纳米粒子，增加了表面面积并增强了固定化探针的结合能力。合成了带有双酚 A（BPA）作为头部基团的硫醇标记长链烃，并将其自组装在金纳米粒子表面上作为传感探针，在结合雌激素受体（ER-α）时呈现出 1 至 30 nM 的线性响应。通过将竞争性结合分析与压电传感器集成，实现了雌酮、17β-雌二醇和 BPA 的检测。在这种检测方案中，不同浓度的 EDCs 与 30 nM 的 ER-α孵育，溶液中未结合的 ER-α被固定在陶瓷谐振器上的探针捕获，从而导致不同 EDCs 的频率变化。生物传感器分析对 EDCs 表现出线性响应，检测限低至 2.4-2.9 nM（S/N=3），并且仅需要 1.5 µl 的小样品体积，测定时间为 2h。还评估了生物传感器分析用于快速简便地测定饮用水和海水中环境相关浓度的 EDCs 的性能，回收率在 94.7%至 109.8%之间。
