Spychalska Kamila, Zając Dorota, Cabaj Joanna
Faculty of Chemistry, Wrocław University of Science and Technology Wybrzeże Wyspiańskiego 27 Wrocław Poland
RSC Adv. 2020 Mar 3;10(15):9079-9087. doi: 10.1039/c9ra09902f. eCollection 2020 Feb 27.
A convenient electrochemical sensing pathway for 17β-estradiol detection was investigated. The system is based on a conducting polymer and horseradish peroxidase (HRP) modified platinum (Pt) electrode. The miniature estradiol biosensor was developed and constructed through the immobilization of HRP in an electroactive surface of the electrode covered with electroconducting polymer - poly(4,7-bis(5-(3,4-ethylenedioxythiophene)thiophen-2-yl)benzothiadiazole). The detection strategy is based on the fact that 17β-estradiol (E2) and pyrocatechol (HQ) are co-substrates for the HRP enzyme. HRP, which does not react with E2, in the presence of HO catalyses the oxidation of HQ to -benzoquinone (Q). With the optimized conditions, such constructed biosensing system demonstrated a convenient level of sensitivity, selectivity in a broad linear range - 0.1 to 200 μM with a detection limit of 105 nM. Furthermore, the method was successfully applied for hormone detection in the presence of potential interfering compounds (ascorbic acid, estriol, estrone, uric acid and cholesterol).
研究了一种用于检测17β-雌二醇的便捷电化学传感途径。该系统基于导电聚合物和辣根过氧化物酶（HRP）修饰的铂（Pt）电极。通过将HRP固定在覆盖有导电聚合物聚（4,7-双（5-（3,4-乙撑二氧噻吩）噻吩-2-基）苯并噻二唑）的电极电活性表面上，开发并构建了微型雌二醇生物传感器。检测策略基于17β-雌二醇（E2）和邻苯二酚（HQ）是HRP酶的共底物这一事实。在HO存在下，不与E2反应的HRP催化HQ氧化为对苯醌（Q）。在优化条件下，如此构建的生物传感系统在0.1至200μM的宽线性范围内表现出便捷的灵敏度和选择性，检测限为105 nM。此外，该方法在存在潜在干扰化合物（抗坏血酸、雌三醇、雌酮、尿酸和胆固醇）的情况下成功应用于激素检测。
