Institute of the Molecular Biology of Plants, CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg Cédex, France.
Theor Appl Genet. 1992 Aug;84(5-6):555-9. doi: 10.1007/BF00224151.
It has been previously shown that in the genome of maize the multiple copies of the histone H3 and H4 multigenic families are organized into eight to ten subfamilies each containing a variable number of copies. Each subfamily is characterized by a specific proximal environment and thus can be revealed by blot-hybridization with its specific 5' probe. Restriction fragment length polymorphism (RFLP) combined with monosomic analysis was used to localize several H3 and H4 subfamilies on maize chromosomes. H3 and H4 genes were found to be located on most, possibly all of the chromosomes, revealing a remarkably dispersed organization of these multigenic families.
先前已经表明，在玉米基因组中，组蛋白 H3 和 H4 多基因家族的多个拷贝被组织成 8 到 10 个亚家族，每个亚家族包含数量不等的拷贝。每个亚家族都有一个特定的近端环境，因此可以通过与特定的 5'探针进行杂交印迹来揭示。限制性片段长度多态性（RFLP）与单体分析相结合，用于将几个 H3 和 H4 亚家族定位在玉米染色体上。发现 H3 和 H4 基因位于大多数，可能是所有的染色体上，这揭示了这些多基因家族的显著分散组织。
