Childs G, Nocente-McGrath C, Lieber T, Holt C, Knowles J A
Cell. 1982 Dec;31(2 Pt 1):383-93. doi: 10.1016/0092-8674(82)90132-5.
We have cloned and characterized members of a small multigene family that encodes late-stage histone H3 and H4 mRNAs from the sea urchin Lytechinus pictus. Unlike their highly repetitive histone gene counterparts, which are expressed at an earlier developmental stage, late H3 and H4 histone genes are not present in tandem repeats. In addition, the late stage H3 and H4 genes are not always tightly clustered together with the H1, H2A and H2B genes as they are in early histone genes. The spacer DNA that separates adjoining H3 and H4 coding regions is not conserved between nonallelic members of the late histone gene family. We have determined the nucleotide sequence of a continuous 2100 bp segment of DNA including both H3 and H4 coding sequences, the entire spacer DNA separating the genes and surrounding nonhistone DNA. The late histone H3 and H4 genes encode proteins identical to their early gene counterparts; however, the 5' leader sequence is shorter in late genes and the codon usage is different.
我们已经克隆并鉴定了一个小多基因家族的成员，该家族编码来自海胆Lytechinus pictus的晚期组蛋白H3和H4 mRNA。与其高度重复的组蛋白基因对应物不同，后者在较早的发育阶段表达，晚期H3和H4组蛋白基因不存在串联重复。此外，晚期H3和H4基因并不总是像早期组蛋白基因那样与H1、H2A和H2B基因紧密聚集在一起。在晚期组蛋白基因家族的非等位成员之间，分隔相邻H3和H4编码区的间隔DNA并不保守。我们已经确定了一段连续2100 bp的DNA片段的核苷酸序列，该片段包括H3和H4编码序列、分隔基因的整个间隔DNA以及周围的非组蛋白DNA。晚期组蛋白H3和H4基因编码的蛋白质与其早期基因对应物相同；然而，晚期基因中的5'前导序列较短，密码子使用情况也不同。
