Kanazin V, Blake T, Shoemaker R C
Department of Agronomy and USDA-ARS-FCR, Iowa State University, Ames,50011, USA.
Mol Gen Genet. 1996 Feb 5;250(2):137-47. doi: 10.1007/BF02174172.
Several variants of the replacement histone H3 genes from soybean, barley and wheat have been cloned and sequenced. Analysis of segregating populations in barley and soybean, as well as analysis of clones isolated from a soybean genomic library, suggested that these genes are dispersed throughout the genome. Several genes contains introns located in similar positions, but of different lengths and sequence. Comparison of mRNA levels in different tissues revealed that the intron-containing and intronless genes have different expression patterns. The distribution of the introns in the histone H3 genes across several plant species suggests that some of the introns might have been lost during the evolution of the gene family. Sequence divergence among introns and gene-flanking sequences in cloned gene variants allowed us to use them as specific probes for localizing individual gene copies and analyzing the genomic distribution of these variants across a range of genotypes.
大豆、大麦和小麦中组蛋白H3替代基因的几个变体已被克隆和测序。对大麦和大豆分离群体的分析，以及对从大豆基因组文库中分离出的克隆的分析表明，这些基因分散在整个基因组中。几个基因含有位于相似位置但长度和序列不同的内含子。不同组织中mRNA水平的比较表明，含内含子和无内含子的基因具有不同的表达模式。组蛋白H3基因内含子在几种植物物种中的分布表明，一些内含子可能在基因家族的进化过程中丢失了。克隆基因变体中内含子和基因侧翼序列之间的序列差异使我们能够将它们用作特异性探针，以定位单个基因拷贝并分析这些变体在一系列基因型中的基因组分布。
