Department of Environmental and Forest Biology College of Envirnmental Science and Forestry, State University of New York, 13210, Syracuse, NY, USA.
Theor Appl Genet. 1992 Aug;84(5-6):617-23. doi: 10.1007/BF00224160.
We have compared copy numbers and blothybridization patterns of histone genes (H3 plus H4) between and within individuals of broad bean (Vicia faba). Copy number differences among individuals in the population of 200 individuals were as great as 27 fold, and as much as 3.2 fold among separate leaves of the same plant. Among F2 progeny from genetic crosses, up to a 5.4-fold range was seen (mean=3.5 fold), and among F1 progeny of self-pollinated plants, up to a 5.9-fold range was observed (mean=2.3 fold). Histone gene blot-hybridization patterns for EcoRI and HindIII were also variable among individuals and indicated that the genes are probably clustered in only a few chromosomal loci. The degree of variation in histone gene copy number per haploid genome (2-55 copies, or 27 fold) was similar to that found previously for ribosomal RNA genes (230-22000, or 95 fold) of V. faba. However, the two gene families change independently, since individuals with a high or low copy number for one gene can have either a high or low copy number for the other. The mechanisms(s) for rapid gene copy number change may be similar for these gene families.
我们比较了蚕豆（Vicia faba）个体间和个体内组蛋白基因（H3 加 H4）的拷贝数和杂交模式。在 200 个个体的群体中，个体之间的拷贝数差异高达 27 倍，同一植物的不同叶片之间的差异高达 3.2 倍。在遗传杂交的 F2 后代中，观察到高达 5.4 倍的范围（平均值为 3.5 倍），在自花授粉植物的 F1 后代中，观察到高达 5.9 倍的范围（平均值为 2.3 倍）。EcoRI 和 HindIII 的组蛋白基因杂交模式在个体之间也存在差异，表明这些基因可能在少数几个染色体位点上聚集。每个单倍体基因组中组蛋白基因拷贝数的变异程度（2-55 个拷贝，或 27 倍）与先前在 V. faba 的核糖体 RNA 基因（230-22000，或 95 倍）中发现的相似。然而，这两个基因家族是独立变化的，因为一个基因的高或低拷贝数的个体可以具有另一个基因的高或低拷贝数。这些基因家族的快速基因拷贝数变化机制可能相似。
