Department of Botany, University of Washington, 98195, Seattle, WA, USA.
Plant Mol Biol. 1986 Sep;6(5):339-45. doi: 10.1007/BF00034941.
Length heterogeneity in the ribosomal repeat of Vicia faba is due to the presence of variable numbers of a 325 bp subrepetitive element within the nontranscribed spacer region. The distribution of size classes among 88 individuals within a population was investigated by blot-hybridization. We find that individual plants can exhibit more than 20 size classes and that hybridization patterns are highly diverse from individual to individual, more so than for any species so far investigated. In contrast, no such differences are observed in patterns for different tissues from a single plant or from parental to F1 generation. Some changes were observed in the F2 generation. We conclude that unequal recombination can give rise to the diversity that we observe for the V. faba rDNA repeats.
蚕豆核糖体基因重复序列的长度不均一性是由于在非转录间隔区存在可变数量的 325bp 亚重复元件。通过杂交印迹分析研究了群体中 88 个个体的大小类分布。我们发现，单个植株可以表现出 20 多种大小类，并且个体之间的杂交模式比迄今为止研究的任何物种都更加多样化。相比之下，来自同一植株的不同组织或亲本到 F1 代的杂交模式则没有这种差异。在 F2 代中观察到了一些变化。我们得出结论，不等交换可以产生我们观察到的蚕豆 rDNA 重复序列的多样性。
