Hilder V A, Livesey R N, Turner P C, Vlad M T
Nucleic Acids Res. 1981 Nov 11;9(21):5737-46. doi: 10.1093/nar/9.21.5737.
We have compared the number of copies of sequences complementary to a cloned Xenopus histone H4 coding sequence in the genomes of Xenopus, Triturus and Ambystoma, amphibian species with widely different C-values (3, 23 and 38pg DNA/haploid genome respectively). Quantitative autoradiography indicates that H4 sequence constitute a greater proportion of the genome the larger that genome is. Measurement of the absolute copy-number by reassociation kinetic analysis indicated 47 +/- 10, 636 +/- 21 2685 +/- 349 copies per haploid genome each in Xenopus, Triturus and Ambystoma respectively. Whilst this confirms a trend of increasing copy-number with increasing C-value, the two are not directly proportional and some other factors must contribute to determining the number of copies of these genes.
我们比较了非洲爪蟾、蝾螈和钝口螈基因组中与克隆的非洲爪蟾组蛋白H4编码序列互补的序列拷贝数,这几种两栖动物的C值差异很大(单倍体基因组的DNA含量分别为3、23和38皮克)。定量放射自显影表明,基因组越大,H4序列在基因组中所占比例越大。通过重缔合动力学分析测量绝对拷贝数,结果表明非洲爪蟾、蝾螈和钝口螈单倍体基因组中每组分别有47±10、636±21和2685±349个拷贝。虽然这证实了拷贝数随C值增加而增加的趋势,但二者并非直接成比例,一定有其他因素影响这些基因的拷贝数。