Plant Cell Research Institute, 6560 Trinity Court, 94568, Dublin, CA, USA.
Plant Cell Rep. 1990 Sep;9(5):285-8. doi: 10.1007/BF00232303.
Callus induction and plantlet regeneration from cotyledonary expiants of sugarbeet was observed utilizing two media formulations, MS and a modified MS termed RVIM both supplemented with 1.0 μg/ml BAP as the sole growth regulator. Callus induction was genotype dependent The USDA line 8787 produced the highest response for callus induction followed by 'Betaseed 4587' and the USDA line C600. This order was conserved on both media formulations. Shoot induction was consistently higher averaging 32% from the RVIM formulation over the 3 genotypes compared to 25% from MS. The antibiotics geneticin, gentamycin, hygromycin, kanamycin and phleomycin were screened with the modified RV system utilizing 'Betaseed 4587'. Callus growth was inhibited by levels of 50 μg/ml geneticin, 150 μg/ml gentamycin, 10 μg/ml hygromycin, 150 μg/ml kanamycin and 20 μg/ml phleomycin. The results indicate that the concentrations of antibiotics used to inhibit callus induction will be sufficient for use as selectable markers in transformation experiments with Beta vulgaris.
利用两种培养基配方（MS 和改良的 MS，称为 RVIM），均添加 1.0μg/ml BAP 作为唯一的生长调节剂，从糖甜菜子叶外植体诱导愈伤组织并再生小植株。愈伤组织诱导依赖于基因型。USDA 8787 品系产生的愈伤组织诱导反应最高，其次是“Betaseed 4587”和 USDA C600 品系。在两种培养基配方上，这个顺序都是保守的。与 MS 相比，利用改良的 RV 系统，利用“Betaseed 4587”筛选了抗生素遗传霉素、庆大霉素、潮霉素、卡那霉素和博莱霉素，再生小植株的诱导率始终更高，平均为 32%，而 3 个基因型的诱导率为 25%。遗传霉素 50μg/ml、庆大霉素 150μg/ml、潮霉素 10μg/ml、卡那霉素 150μg/ml 和博莱霉素 20μg/ml 抑制愈伤组织生长。结果表明，用于抑制愈伤组织诱导的抗生素浓度将足以作为转化实验中选择标记物，用于转化糖用甜菜。
