Instituut voor Moleculaire Biologie, Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, Paardenstraat 65, B-1640, St-Genesius-Rode, Belgium.
Plant Cell Rep. 1985 Apr;4(2):66-9. doi: 10.1007/BF00269208.
A systematic procedure is described to obtain habituated cell suspensions from different explant sources of sugarbeet (Beta vulgaris L.).Cytokinin habituation occurred spontaneously. Auxin habituation could be induced by successive lowering of the external hormone concentration. Habituation was reversible and independent of the cell type or explant source. The habituated nature of the cells was lost during regeneration and had to be reinduced by adequate treatments.A method for initiating habituated self-regenerating lines was established. These cultures were less stable. Total dedifferentiation caused the loss of the regeneration capacity. Reculturing of the regenerated plants did not result in a higher percentage of primary regeneration.
描述了从不同的甜菜（Beta vulgaris L.）外植体来源获得适应细胞悬浮液的系统程序。细胞分裂素适应是自发发生的。通过连续降低外源性激素浓度可以诱导生长素适应。适应是可逆的，与细胞类型或外植体来源无关。细胞的适应特性在再生过程中丧失，必须通过适当的处理重新诱导。建立了启动适应自再生系的方法。这些培养物不太稳定。总去分化导致再生能力丧失。再生植物的再培养不会导致更高比例的原生再生。
