Project-group Plant Cell Biotechnology, Division of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, P.O. Box 9502, NL-2300, RA Leiden, The Netherlands.
Plant Cell Rep. 1993 Aug;12(10):573-6. doi: 10.1007/BF00233063.
The subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus roseus (L.) G. Don and Tabernaemontana divaricata (L.) R. Br. ex Roem. et Schult, was investigated. It was found that tryptophan decarboxylase is an extra-vacuolar enzyme, whereas strictosidine synthase is active inside the vacuole. Strong indications were obtained for the localization of strictosidine glucosidase on the outside of the tonoplast. The results suggest that tryptamine is transported into the vacuole where it is condensed with secologanin to form strictosidine, and that strictosidine passes the tonoplast and is subsequently hydrolysed outside the vacuole.
色氨酸脱羧酶、文多灵合酶和文多灵葡萄糖苷酶在长春花（L.）G. Don 和Tabernaemontana divaricata（L.）R. Br. ex Roem. 和 Schult 的悬浮培养细胞中的亚细胞定位进行了研究。发现色氨酸脱羧酶是一种细胞外酶，而文多灵合酶在液泡内活跃。在液泡膜的外侧有强烈的迹象表明存在文多灵葡萄糖苷酶。研究结果表明，色胺被运送到液泡中，在那里与 secologanin 缩合形成文多灵，文多灵穿过液泡膜，随后在液泡外被水解。
