Institute for Biochemistry of Plants, Heinrich Heine University Düsseldorf, Universitätsstrasse 1, D-4000, Düsseldorf 1, Germany.
Planta. 1990 Feb;180(3):383-9. doi: 10.1007/BF00198790.
Spinach (Spinacia oleracea L.) plants were acclimated to 1° C or maintained at 18° C under the same light regime (260-300 μmol photons·m(-2)·s(-1)). The cold acclimation led to several metabolic and biochemical changes that apparently include improved protection of the photosynthetic apparatus against active oxygen species. In particular, cold-acclimated leaves exhibited a considerably higher ascorbate content and significantly increased activities of superoxide dismutase, ascorbate peroxidase, and monodehydroascorbate reductase in the chloroplasts. The level of dehydroascorbate reductase did not alter. Catalase activity decreased. The photosynthetic pigment composition of cold-acclimated spinach was characterized by increased levels of the xanthophylls lutein + zeaxanthin and violaxanthin. The observed changes are discussed in terms of their possible relevance for plant resistance to photoinhibition at chilling temperatures.
菠菜（Spinacia oleracea L.）植株在 1°C 下适应或在相同光照条件下（260-300 μmol 光子·m(-2)·s(-1))保持在 18°C。低温驯化导致了几种代谢和生化变化，显然包括改善对光合作用器官对活性氧的保护。特别是，低温驯化的叶片表现出相当高的抗坏血酸含量，并且叶绿体中超氧化物歧化酶、抗坏血酸过氧化物酶和单脱氢抗坏血酸还原酶的活性显著增加。脱氢抗坏血酸还原酶的水平没有改变。过氧化氢酶活性降低。低温驯化的菠菜的光合色素组成的特征是叶黄素+玉米黄质和紫黄质的水平增加。观察到的变化将根据它们对植物在低温下对光抑制的抗性的可能相关性进行讨论。
