Chowrira G M, Akella V, Lurquin P F
Department of Genetics and Cell Biology, Washington State University, Pullman 99164-4234, USA.
Mol Biotechnol. 1995 Feb;3(1):17-23. doi: 10.1007/BF02821331.
Transient expression and stable integration and expression of transgenes were observed in the tissues and offspring of certain leguminous plants after electroporation of DNA into intact nodal meristems in planta. The method described in this article thus allows the study of transgene expression in tissues differentiating from meristematic cells present in the treated buds. In addition, transgenic plants can be recovered in the offspring of electroporated individuals. Therefore, this technique allows the production of transgenic leguminous plants without the need for in vitro tissue culture, often a major hurdle with this family.
在将DNA电穿孔导入完整植株的节间分生组织后，在某些豆科植物的组织和后代中观察到了转基因的瞬时表达、稳定整合和表达。因此，本文所述方法能够研究在处理过的芽中存在的分生细胞分化而来的组织中的转基因表达。此外，在电穿孔处理个体的后代中可以获得转基因植株。因此，这项技术无需进行体外组织培养就能培育转基因豆科植物，而体外组织培养往往是该植物家族面临的一个主要障碍。
