Bruno John G
Nanohmics Inc., 6201 E. Oltorf Street, Suite 400, Austin, TX, 78741, USA.
J Fluoresc. 2024 Nov 22. doi: 10.1007/s10895-024-04049-1.
Initial proof-of-concept development of competitive lateral flow (LF) test strips involving red quantum dots (Qdots) is demonstrated with visible confirmation of the competitive displacement of Qdot-protein-saxitoxin (Stx) and -tetrodotoxin (Ttx) conjugates over 15 min. The measured limits of detection (LODs) for the best versions of the assays were in the 1-2 µg range when a bovine serum albumin (BSA)-Stx or -Ttx conjugate was used as the analyte for safety reasons and NIH ImageJ image analysis was applied. However, when one assumes that only one of the primary amines in BSA labeled with Stx or Ttx is needed as the "epitope" for capture and calculates the weight of the toxin adducts on an equal mole basis to that of 1 µg of toxin-BSA conjugate, the LODs are as low as 4.5 ng of toxin. No cross-reactivity was seen for these prototype test strips when BSA-Stx and BSA-Ttx were switched as analytes at or near their respective LODs which is in agreement with published specificity and affinity studies for these aptamers in the literature.
展示了涉及红色量子点（Qdots）的竞争性侧向流动（LF）测试条的初步概念验证开发，在15分钟内可见量子点 - 蛋白质 - 石房蛤毒素（Stx）和 - 河豚毒素（Ttx）缀合物的竞争性取代。出于安全原因，当使用牛血清白蛋白（BSA）-Stx或 -Ttx缀合物作为分析物并应用NIH ImageJ图像分析时，该检测方法最佳版本的测量检测限（LOD）在1-2μg范围内。然而，当假设用Stx或Ttx标记的BSA中只有一种伯胺作为捕获的“表位”，并以与1μg毒素 - BSA缀合物等摩尔的基础计算毒素加合物的重量时，LOD低至4.5ng毒素。当BSA-Stx和BSA-Ttx在其各自的LOD或接近LOD处作为分析物切换时，这些原型测试条没有观察到交叉反应，这与文献中针对这些适体发表的特异性和亲和力研究一致。
