Leigh M W, Gambling T M, Carson J L, Collier A M, Wood R E, Boat T F
Exp Lung Res. 1986;10(2):153-69. doi: 10.3109/01902148609061490.
We explored the usefulness of the postnatal ferret as a model for early developmental events in the large airways, using light and scanning electron microscopy. In the first 28 postnatal days, ferret tracheal surface epithelium and glands undergo dramatic growth and development. Tracheal surface area increases 8-fold. At birth, ciliated cells are sparse (9.4 +/- 1.2% of total epithelial cells). A significant increase in ciliated cells is observed at weekly intervals and by day 28 the ciliated cell is the predominant cell type (54.2 +/- 2.8% of total epithelial cells). Secretory cells decrease from 66.4 +/- 1.0% at birth to 22.2 +/- 2.8% of total epithelial cells. Histochemical staining of the granules of the epithelial secretory cells changes from predominantly non-acidic (staining with PAS but not Alcian blue) to predominantly acidic (staining also with Alcian blue). During the same time interval, tracheal glands develop from intraepithelial cellular aggregates devoid of secretory granules at birth into complex, submucosal tubuloacinar structures composed predominantly of cells containing non-acidic secretory granules at 28 days. Therefore, infant ferrets offer an opportunity to examine the structural and functional components of the mucociliary clearance mechanism at developmental stages which occur prenatally in many laboratory animals and in humans.
我们利用光学显微镜和扫描电子显微镜，探讨了产后雪貂作为大气道早期发育事件模型的实用性。在出生后的前28天，雪貂气管表面上皮和腺体经历了显著的生长和发育。气管表面积增加了8倍。出生时，纤毛细胞稀少（占上皮细胞总数的9.4±1.2%）。每周观察到纤毛细胞显著增加，到第28天时，纤毛细胞成为主要细胞类型（占上皮细胞总数的54.2±2.8%）。分泌细胞从出生时的66.4±1.0%减少到上皮细胞总数的22.2±2.8%。上皮分泌细胞颗粒的组织化学染色从主要非酸性（用PAS染色但不用阿尔辛蓝染色）变为主要酸性（也用阿尔辛蓝染色）。在同一时间间隔内，气管腺从出生时无分泌颗粒的上皮内细胞聚集体发育成28天时主要由含非酸性分泌颗粒的细胞组成的复杂的黏膜下管状腺泡结构。因此，幼年雪貂为研究在许多实验动物和人类中发生于产前的发育阶段的黏液纤毛清除机制的结构和功能成分提供了一个机会。
