Trifilieff E, Luu B, Nussbaum J L, Roussel G, Espinosa de los Monteros A, Sabatier J M, Van Rietschoten J
FEBS Lett. 1986 Mar 31;198(2):235-9. doi: 10.1016/0014-5793(86)80412-4.
Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cross react with DM-20, while both proteolipids had been shown previously to be recognized by antibodies directed against the C-terminal hexapeptide of MMPL. This is in accordance with previous findings showing that DM-20 differs only from MMPL by a deletion of residues 100-140 (+/- few units). Furthermore, this site-specific immunological probe also recognizes MMPL in its native form in oligodendrocytes in primary glial cell cultures.
主要髓磷脂蛋白脂蛋白（MMPL，也称为PLP）和DM-20是中枢神经系统髓磷脂的两种主要内在膜蛋白。通过针对MMPL的合成十三肽117-129产生抗体，获得了一种针对MMPL的特异性免疫探针。针对该肽的抗体与MMPL发生反应，但不与DM-20发生交叉反应，而这两种蛋白脂质先前已被证明可被针对MMPL C端六肽的抗体识别。这与先前的研究结果一致，即DM-20与MMPL的区别仅在于缺失了100-140位残基（±几个单位）。此外，这种位点特异性免疫探针还能识别原代神经胶质细胞培养物中少突胶质细胞内天然形式的MMPL。
