Bruce M G, Ferguson A
Immunology. 1986 Apr;57(4):627-30.
Suppression of systemic immunity after the feeding of antigen was investigated in mice by means of serum transfer experiments. Serum collected from mice 1 hr after a single intragastric dose of 25 mg OVA induced suppression of systemic DTH when injected intraperitoneally into recipient mice. This suppression was found to be restricted to the cell-mediated limb of immunity and was antigen-specific. A postulated function of the intestine, conversion of antigen into tolerogenic form by means of intestinal antigen processing, was studied by attempting to mimic intestinal alteration of OVA by chemical modification of the antigen. Parenteral injection of mice with either deaggregated or denatured OVA did not produce the typical pattern of unresponsiveness seen in animals given intestinally processed OVA. Intestinal processing was also shown to be distinct from systemic antigen processing. Mice injected with serum containing systemically 'filtered' OVA did not become tolerant to OVA in the manner of recipients of serum from OVA-fed mice.
通过血清转移实验在小鼠中研究了喂食抗原后全身免疫的抑制情况。单次胃内给予25mg卵清蛋白（OVA）1小时后从小鼠收集的血清，当腹腔注射到受体小鼠体内时可诱导全身迟发型超敏反应（DTH）的抑制。发现这种抑制仅限于免疫的细胞介导分支，并且具有抗原特异性。通过尝试通过抗原的化学修饰模拟OVA的肠道改变，研究了肠道的一种假定功能，即将抗原转化为致耐受性形式。给小鼠皮下注射解聚或变性的OVA不会产生在给予经肠道处理的OVA的动物中所见的典型无反应模式。肠道处理也显示与全身抗原处理不同。注射含有经全身“过滤”的OVA的血清的小鼠不会像喂食OVA的小鼠血清的受体那样对OVA产生耐受性。
