肠道处理对小鼠体内吸收的循环卵清蛋白的免疫原性和分子大小的影响。
The influence of intestinal processing on the immunogenicity and molecular size of absorbed, circulating ovalbumin in mice.
作者信息
Bruce M G, Ferguson A
出版信息
Immunology. 1986 Oct;59(2):295-300.
The presence of a tolerogen in mouse serum within 1 hr of antigen feeding prompted further study. Therefore, serum from mice fed with ovalbumin (OVA) was subjected to various immunochemical and biological tests. The appearance of tolerogen in serum was concomitant with the presence of OVA detected by a specific ELISA. Absorption of tolerogenic serum with anti-OVA antibody coupled to Sepharose beads effectively removed the tolerogenic moiety from the serum and confirmed that not only was tolerogenicity associated with the presence of antigen, but that binding sites for antibody were intact on this tolerogenic form of OVA. Finally, serum fractions from antigen-fed mice were assayed for total protein content, ELISA-detectable OVA and in vivo effect on systemic immunity. The only serum fraction in which immunoreactive OVA was detected contained proteins close to the molecular weight of native OVA and induced significant immune suppression in recipients. Serum fractions lacking immunoreactive OVA were not significantly tolerogenic in vivo. These experiments confirm that when OVA is absorbed across the gut mucosa it is subtly altered into a tolerogenic form. The recognition of gut-processed OVA by T-suppressor cells is discussed.
喂食抗原后1小时内小鼠血清中存在耐受原，这促使了进一步研究。因此，给喂食卵清蛋白（OVA）的小鼠的血清进行了各种免疫化学和生物学检测。血清中耐受原的出现与通过特异性ELISA检测到的OVA的存在同时发生。用偶联到琼脂糖珠上的抗OVA抗体吸收耐受血清有效地从血清中去除了耐受部分，并证实不仅耐受性与抗原的存在相关，而且在这种OVA的耐受形式上抗体结合位点是完整的。最后，对抗原喂食小鼠的血清组分进行总蛋白含量、ELISA可检测的OVA以及对全身免疫的体内效应分析。唯一检测到免疫反应性OVA的血清组分含有接近天然OVA分子量的蛋白质，并在受体中诱导了显著的免疫抑制。缺乏免疫反应性OVA的血清组分在体内没有显著的耐受性。这些实验证实，当OVA通过肠道黏膜吸收时，它会微妙地转变为一种耐受形式。还讨论了T抑制细胞对肠道加工的OVA的识别。
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