Key Laboratory for Integrated Regulation and Resources Development on Shallow Lakes, China Ministry of Education, College of Environment, Hohai University, 1 Xikang Road, 210098 Nanjing, China.
Key Laboratory for Integrated Regulation and Resources Development on Shallow Lakes, China Ministry of Education, College of Environment, Hohai University, 1 Xikang Road, 210098 Nanjing, China.
Chemosphere. 2014 Mar;99:102-8. doi: 10.1016/j.chemosphere.2013.10.036. Epub 2013 Nov 8.
To investigate the distribution, bioconcentration, metabolism, and biomarker responses of macrolide antibiotic roxithromycin (ROX) in fish, crucian carp (Carassius auratus) were exposed to various concentrations of ROX (4, 20, and 100μgL(-1)) for 20d. The ROX content in different tissues was quantified using UPLC/MS/MS. The liver exhibited the highest ROX concentration followed by the bile, gills, and muscle tissues. After 15d of exposure to different concentrations of ROX, the bioconcentration factors were 2.15-38.0 in the liver, 0.950-20.7 in the bile, 0.0506-19.7 in the gill, and 0.0439-13.8 in the muscle; these results were comparable to the estimated BCF values. The metabolites formed in the bile were identified based on metabolic identification in human bile. Additionally, the biomarkers, including acetylcholinesterase in the brain, as well as 7-ethoxyresorufin O-deethylase and superoxide dismutase in the liver changed significantly after 5, 10, 15, and 20d of exposure (P<0.05). Our results suggest that ROX can accumulate and be metabolized in fish; therefore, interactions between ROX or its metabolites and the biological systems may induce biochemical disturbances in fish.
为了研究大环内酯类抗生素罗红霉素（ROX）在鱼类中的分布、生物富集、代谢和生物标志物反应，将鲫鱼（Carassius auratus）暴露于不同浓度的 ROX（4、20 和 100μg/L）中 20 天。使用 UPLC/MS/MS 定量测定不同组织中的 ROX 含量。肝脏中 ROX 浓度最高，其次是胆汁、鳃和肌肉组织。暴露于不同浓度的 ROX 15 天后，肝脏中的生物浓缩因子为 2.15-38.0，胆汁中的为 0.950-20.7，鳃中的为 0.0506-19.7，肌肉中的为 0.0439-13.8；这些结果与估计的 BCF 值相当。根据人胆汁中的代谢鉴定，鉴定出胆汁中形成的代谢物。此外，暴露 5、10、15 和 20 天后，大脑中的乙酰胆碱酯酶以及肝脏中的 7-乙氧基resorufin O-去乙基酶和超氧化物歧化酶等生物标志物发生了显著变化（P<0.05）。我们的结果表明，ROX 可以在鱼类中积累和代谢；因此，ROX 或其代谢物与生物系统之间的相互作用可能会在鱼类中引起生化紊乱。
