Lhoste E, Aprahamian M, Pousse A, Hoeltzel A, Stock-Damge C
Peptides. 1985;6 Suppl 3:89-97. doi: 10.1016/0196-9781(85)90356-0.
This work investigates the effect, on the rat pancreas, of a chronic administration of bombesin in function of the dose and duration of treatment and examines whether this effect may be mediated by the release of endogenous gastrin or cholecystokinin. Bombesin, administered three times daily for 5 or 15 days, induced a marked increase in pancreatic weight, its protein, RNA and enzyme contents with the dose of 10 micrograms/kg body weight; the ratios of pancreatic weight, protein and RNA contents to DNA contents increased significantly after a 5 day treatment, suggesting cellular hypertrophy. Pancreatic DNA content was markedly enhanced after a 15 day treatment, suggesting cellular hyperplasia. Antrectomy decreased plasma gastrin levels, but did not alter the pancreatico-trophic action of a 10 micrograms/kg bombesin treatment for 5 days. Proglumide, an inhibitor of cholecystokinin and gastrin in the pancreas, did not affect the growth of the pancreas induced by a 10 micrograms/kg bombesin treatment for 5 days. It is concluded that chronic bombesin induces, in the rat pancreas, cellular hypertrophy or hyperplasia depending on the duration of treatment. Pancreatic hypertrophy is not mediated by the release of endogenous gastrin or cholecystokinin.
本研究探讨了长期给予蛙皮素对大鼠胰腺的影响，该影响取决于给药剂量和疗程，并研究了这种影响是否可能由内源性胃泌素或胆囊收缩素的释放介导。每天三次给予蛙皮素，持续5天或15天，剂量为10微克/千克体重时，可使胰腺重量、蛋白质、RNA和酶含量显著增加；5天治疗后，胰腺重量、蛋白质和RNA含量与DNA含量的比值显著增加，提示细胞肥大。15天治疗后，胰腺DNA含量显著增加，提示细胞增生。胃窦切除术可降低血浆胃泌素水平，但不改变10微克/千克蛙皮素5天治疗的促胰腺生长作用。丙谷胺是胰腺中胆囊收缩素和胃泌素的抑制剂，不影响10微克/千克蛙皮素5天治疗诱导的胰腺生长。结论是，长期给予蛙皮素可使大鼠胰腺根据治疗持续时间发生细胞肥大或增生。胰腺肥大不是由内源性胃泌素或胆囊收缩素的释放介导的。
