Department of Plant Biology (Plant Physiology), University of Murcia, Espinardo, E-30071, Murcia, Spain.
Plant Cell Rep. 1992 Oct;11(11):592-6. doi: 10.1007/BF00233099.
The accumulation of both neohesperidin and naringin as major flavonoids in callus cultures of bitter orange (Citrus aurantium) was demonstrated using high performance liquid chromatography with a diode-array detector. The identity of both compounds was confirmed by their corresponding nuclear magnetic resonance spectra. The levels of neohesperidin are higher than those of naringin in callus culture, as they are in immature fruit, and high concentrations of both are found in young tissues such as immature fruits and the outer zone of calli.
采用二极管阵列检测器的高效液相色谱法显示，在枳（Citrus aurantium）愈伤组织中，新橙皮苷和柚皮苷作为主要类黄酮积累。通过相应的核磁共振谱证实了这两种化合物的身份。在愈伤组织中，新橙皮苷的含量高于柚皮苷，就像在未成熟的果实中一样，而且在未成熟的果实和愈伤组织的外区等年轻组织中，两者的浓度都很高。
