体外繁殖优质紫檀（Dalbergia latifolia Roxb.）。

In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb.).

机构信息

Plant Tissue Culture Laboratory, Department of Microbiology and Cell Biology, Indian Institute of Science, 560 012, Bangalore, India.

出版信息

Plant Cell Rep. 1992 Apr;11(3):126-31. doi: 10.1007/BF00232164.

DOI:10.1007/BF00232164

PMID:24213544

Abstract

Induction of single and multiple shoots was obtained from nodal expiants of 60-80 year-old elite trees of rosewood on Murashige and Skoog's basal medium supplemented with 6-benzylaminopurine (1.0 mg 1(-1)) and δ -Naphthalene acetic acid (0.05 mg 1(-1)) or indole acetic acid (0.5 mg 1(-1)). Multiplication of shoots was obtained on MS (reduced major elements) or Woody Plant Medium supplemented with 6-benzylaminopurine (1.0 mg 1(-1)) and kinetin (0.5-1.0 mg 1(-1)). Excised shoots were rooted on half-strength MS with IBA (2.0 mg 1(-1)) to obtain complete plantlets. The regenerated plantlets have been acclimatized and successfully transferred to the soil.

摘要

从 60-80 年生紫檀老优树上的节点外植体诱导出单芽和多芽，在 Murashige 和 Skoog 的基本培养基上添加 6-苄基氨基嘌呤（1.0 mg 1(-1)) 和 δ -萘乙酸（0.05 mg 1(-1)) 或吲哚乙酸（0.5 mg 1(-1))。在 MS（减少主要元素）或 Woody Plant Medium 上添加 6-苄基氨基嘌呤（1.0 mg 1(-1)) 和激动素（0.5-1.0 mg 1(-1)) 可获得芽的增殖。将外植体剪下后在含有 IBA（2.0 mg 1(-1)) 的半强度 MS 上生根，以获得完整的小植株。再生小植株已适应环境并成功移栽到土壤中。