Sha Valli Khan P S, Prakash E, Rao K R
Plant Tissue Culture Lab., Department of Botany, Sri Venkateswara University, 517 503, Tirupati, India.
Plant Cell Rep. 1997 Feb;16(5):325-328. doi: 10.1007/BF01088290.
Shoot proliferation was obtained from shoot tip and nodal bud explants ofSyzygium alternifolium (Wight) walp on modified Murashige and Skoogs medium (MS) supplemented with either BA, KN or AD alone or BA in combination with either IAA, NAA or IBA. A combination of BA and auxins produced more shoots from both types of explants than on the medium containing only cytokinins. The highest multiplication rate was achieved with nodal bud explants in presence of 17.7μM BA and 2.6μM NAA. Excised shoots were rooted on half-strength MS medium with either IAA or IBA. The regenerated plantlets have been successfully acclimatized and transferred to soil. About 70% of plantlets have survived underex vitro conditions.
在添加了单独的BA、KN或AD，或BA与IAA、NAA或IBA组合的改良Murashige和Skoogs培养基（MS）上，从互生蒲桃（Syzygium alternifolium (Wight) walp）的茎尖和节间芽外植体获得了芽增殖。与仅含有细胞分裂素的培养基相比，BA和生长素的组合从两种外植体产生了更多的芽。在17.7μM BA和2.6μM NAA存在下，节间芽外植体实现了最高的增殖率。切下的芽在含有IAA或IBA的1/2强度MS培养基上生根。再生的小植株已成功驯化并转移到土壤中。约70%的小植株在离体条件下存活。
