母体补充乳铁蛋白对生长和脑代谢的保护作用。

Protective effects of maternal nutritional supplementation with lactoferrin on growth and brain metabolism.

作者信息

Somm Emmanuel, Larvaron Pierre, van de Looij Yohan, Toulotte Audrey, Chatagner Alexandra, Faure Magali, Métairon Sylviane, Mansourian Robert, Raymond Frédéric, Gruetter Rolf, Wang Bing, Sizonenko Stéphane V, Hüppi Petra S

机构信息

Department of Paediatrics, Division of Development and Growth, University of Geneva School of Medicine, Geneva, Switzerland.

1] Department of Paediatrics, Division of Development and Growth, University of Geneva School of Medicine, Geneva, Switzerland [2] Laboratory for Functional and Metabolic Imaging, E´cole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

出版信息

Pediatr Res. 2014 Jan;75(1-1):51-61. doi: 10.1038/pr.2013.199. Epub 2013 Nov 8.

DOI:10.1038/pr.2013.199

PMID:24213624

Abstract

BACKGROUND

Intrauterine growth restriction (IUGR) is a major risk factor for both perinatal and long-term morbidity. Bovine lactoferrin (bLf) is a major milk glycoprotein considered as a pleiotropic functional nutrient. The impact of maternal supplementation with bLf on IUGR-induced sequelae, including inadequate growth and altered cerebral development, remains unknown.

METHODS

IUGR was induced through maternal dexamethasone infusion (100 μg/kg during last gestational week) in rats. Maternal supplementation with bLf (0.85% in food pellet) was provided during both gestation and lactation. Pup growth was monitored, and Pup brain metabolism and gene expression were studied using in vivo (1)H NMR spectroscopy, quantitative PCR, and microarray in the hippocampus at postnatal day (PND)7.

RESULTS

Maternal bLf supplementation did not change gestational weight but increased the birth body weight of control pups (4%) with no effect on the IUGR pups. Maternal bLf supplementation allowed IUGR pups to recover a normalized weight at PND21 (weaning) improving catch-up growth. Significantly altered levels of brain metabolites (γ-aminobutyric acid, glutamate, N-acetylaspartate, and N-acetylaspartylglutamate) and transcripts (brain-derived neurotrophic factor (BDNF), divalent metal transporter 1 (DMT-1), and glutamate receptors) in IUGR pups were normalized with maternal bLf supplementation.

CONCLUSION

Our data suggest that maternal bLf supplementation is a beneficial nutritional intervention able to revert some of the IUGR-induced sequelae, including brain hippocampal changes.

摘要

背景

宫内生长受限（IUGR）是围产期和长期发病的主要危险因素。牛乳铁蛋白（bLf）是一种主要的乳类糖蛋白，被认为是一种具有多种功能的营养物质。母体补充bLf对IUGR引起的后遗症（包括生长不足和脑发育改变）的影响尚不清楚。

方法

通过在大鼠妊娠最后一周静脉注射地塞米松（100μg/kg）诱导IUGR。在妊娠和哺乳期母体补充bLf（食物颗粒中含0.85%）。监测幼崽生长情况，并在出生后第7天（PND7）使用体内氢核磁共振波谱、定量PCR和海马微阵列研究幼崽脑代谢和基因表达。

结果

母体补充bLf未改变妊娠体重，但增加了对照幼崽的出生体重（4%），对IUGR幼崽无影响。母体补充bLf使IUGR幼崽在PND21（断奶）时恢复正常体重，促进追赶生长。母体补充bLf可使IUGR幼崽脑代谢物（γ-氨基丁酸、谷氨酸、N-乙酰天门冬氨酸和N-乙酰天门冬氨酰谷氨酸）和转录物（脑源性神经营养因子（BDNF）、二价金属转运蛋白1（DMT-1）和谷氨酸受体）的显著改变水平恢复正常。