Volckova M, Linhartova P Borilova, Trefna T, Vlazny J, Musilova K, Kukletova M, Kukla L, Holla L Izakovicova
Clinic of Stomatology, St. Anne's Faculty Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Caries Res. 2014;48(1):39-44. doi: 10.1159/000351689. Epub 2013 Nov 6.
Dental caries is a complex, multifactorial disease and one of the most common illnesses worldwide. Its etiology is related to microbial, dietary and host factors. Recent evidence suggests a role of lactotransferrin (LTF) in caries. The purpose of this study was to determine the association between LTF gene polymorphism and dental caries.
In this case-control study, 637 unrelated children, aged 11-13 years, were enrolled. The subjects were divided into two groups, i.e. caries-free (decayed/missing/filled teeth = 0) and caries-affected children (decayed/missing/filled teeth ≥ 1). The LTF rs1126478 (140A/G in exon 2, Lys/Arg) genotypes were determined by PCR with restriction analysis using the EarI enzyme.
Of 637 children, 155 (24.3%) were caries free. There were no statistically significant differences between caries levels and allele or genotype distributions in the total cohort. When the caries-affected group (n = 482) was stratified into low (decayed/missing/filled teeth = 1), moderate (2 ≤ decayed/missing/filled teeth ≤ 3) and high (decayed/missing/filled teeth ≥ 4) caries experience, allele and genotype frequencies were similar among all subgroups.
The LTF 140A/G (exon 2, Lys/Arg) polymorphism was not associated with the susceptibility to or severity of dental caries in the Czech population.
龋齿是一种复杂的多因素疾病，也是全球最常见的疾病之一。其病因与微生物、饮食和宿主因素有关。最近的证据表明乳铁蛋白（LTF）在龋齿中起作用。本研究的目的是确定LTF基因多态性与龋齿之间的关联。
在这项病例对照研究中，纳入了637名11至13岁的无亲缘关系儿童。受试者分为两组，即无龋儿童（龋失补牙数=0）和患龋儿童（龋失补牙数≥1）。通过使用EarI酶的PCR限制性分析确定LTF rs1126478（外显子2中的140A/G，赖氨酸/精氨酸）基因型。
637名儿童中，155名（24.3%）无龋。在整个队列中，龋病水平与等位基因或基因型分布之间无统计学显著差异。当将患龋组（n = 482）分为低龋（龋失补牙数=1）、中度龋（2≤龋失补牙数≤3）和高龋（龋失补牙数≥4）经历时，所有亚组的等位基因和基因型频率相似。
在捷克人群中，LTF 140A/G（外显子2，赖氨酸/精氨酸）多态性与龋齿的易感性或严重程度无关。
