Borilova Linhartova Petra, Kastovsky Jakub, Bartosova Michaela, Musilova Kristina, Zackova Lenka, Kukletova Martina, Kukla Lubomir, Izakovicova Holla Lydie
Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Masaryk University, Brno, Czech Republic.
Caries Res. 2016;50(2):89-96. doi: 10.1159/000443534. Epub 2016 Feb 27.
Dental caries is a multifactorial, infectious disease where genetic predisposition plays an important role. Insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) has very recently been associated with caries in Polish children. The aim of this study was to analyze ACE I/D polymorphism in a group of caries-free children versus subjects affected by dental caries in the Czech population.
In this case-control study, 182 caries-free children (with decayed/missing/filled teeth, DMFT = 0), 561 subjects with dental caries (DMFT ≥1) aged 13-15 years and 220 children aged 2-6 years with early childhood caries (ECC, dmft ≥1) were included. Genotype determination of ACE I/D polymorphism in intron 16 was based on the TaqMan method.
Although no significant differences in the allele or genotype frequencies between the caries-free children and those affected by dental caries were observed, statistically significant differences between the children with DMFT = 0 and the subgroup of 179 patients with high caries experience (DMFT ≥4; p < 0.01 and p < 0.05, respectively) were detected. The comparison of DD versus II+ID genotype frequencies between the patients with DMFT ≥1 or DMFT ≥4 and healthy children also showed significant differences (31.5% or 35.6% vs. 23.6%, p < 0.05 or p < 0.01, respectively). A gender-based analysis identified a significant difference in the DD versus II+ID genotype frequencies only in girls (p < 0.05). In contrast, no significant association of ACE I/D polymorphism with ECC in young children was found (p > 0.05).
ACE I/D polymorphism may be associated with caries in permanent but not primary dentition, especially in girls in the Czech population.
龋齿是一种多因素的感染性疾病，遗传易感性在其中起着重要作用。血管紧张素转换酶（ACE）插入/缺失（I/D）多态性最近被发现与波兰儿童的龋齿有关。本研究的目的是分析捷克人群中一组无龋儿童与患龋儿童的ACE I/D多态性。
在这项病例对照研究中，纳入了182名无龋儿童（龋失补牙数，DMFT = 0）、561名年龄在13至15岁的患龋受试者（DMFT≥1）以及220名患有幼儿龋（ECC，乳牙龋失补牙数≥1）的2至6岁儿童。基于TaqMan方法对第16内含子中的ACE I/D多态性进行基因型测定。
虽然未观察到无龋儿童与患龋儿童之间等位基因或基因型频率的显著差异，但在DMFT = 0的儿童与179名高龋经验患者亚组（DMFT≥4；分别为p < 0.01和p < 0.05）之间检测到统计学上的显著差异。DMFT≥1或DMFT≥4的患者与健康儿童之间DD与II + ID基因型频率的比较也显示出显著差异（分别为31.5%或35.6%对23.6%，p < 0.05或p < 0.01）。基于性别的分析仅在女孩中发现DD与II + ID基因型频率存在显著差异（p < 0.05）。相比之下，未发现ACE I/D多态性与幼儿ECC之间存在显著关联（p > 0.05）。
ACE I/D多态性可能与恒牙而非乳牙的龋齿有关，尤其是在捷克人群的女孩中。
