Moazzez R V, Austin R S, Rojas-Serrano M, Carpenter G, Cotroneo E, Proctor G, Zaidel L, Bartlett D W
Salivary Research Unit, King's College London Dental Institute, London, UK.
Caries Res. 2014;48(1):57-62. doi: 10.1159/000352042. Epub 2013 Nov 6.
The acquired pellicle adheres to tooth surfaces and has been suggested to provide differing degrees of protection against acidic erosion. This study investigated whether pellicle formed on enamel blocks in patients suffering dietary dental erosion modified the effect of an in vitro simulated dietary challenge, in comparison with pellicle formed on enamel blocks in healthy subjects and to no-pellicle enamel samples. Sixty subjects recruited from dental erosion clinics were compared to healthy age-matched controls. Subjects wore a custom-made maxillary splint holding human enamel blocks for 1 h during which the acquired enamel pellicle was formed. Enamel blocks were removed from the splints and a simulated dietary erosive challenge of 10 min was performed. In addition the challenge was performed on 30 enamel samples without pellicle. Profilometry showed no statistical difference between samples from the erosion subjects with a mean step height of 1.74 µm (SD 0.88) and median roughness (Sa) of 0.39 µm (interquartile range, IQR 0.3-0.56) and the controls with 1.34 µm (SD 0.66) and 0.33 µm (IQR 0.27-0.38), respectively. The control samples without pellicle had Sa of 0.44 µm (IQR 0.36-0.69) and these differences were statistically significant compared to those from the healthy subjects (p = 0.002). Mean (SD) microhardness reduction with a 100-gram load for the erosion group was 113.5 (10) KHN, for healthy subjects was 93 (15.4) KHN and for the enamel samples without pellicle 139.6 (21.8) KHN and all groups were statistically different. The microhardness and roughness data suggested the pellicle influenced erosion under these study conditions.
获得性薄膜附着于牙齿表面，有人认为它能提供不同程度的抗酸蚀保护。本研究调查了饮食性牙侵蚀患者牙釉质块上形成的薄膜，与健康受试者牙釉质块上形成的薄膜以及无薄膜的牙釉质样本相比，是否会改变体外模拟饮食挑战的效果。从牙侵蚀诊所招募的60名受试者与年龄匹配的健康对照组进行比较。受试者佩戴定制的上颌夹板，其中夹着人类牙釉质块1小时，在此期间形成获得性牙釉质薄膜。将牙釉质块从夹板上取下，进行10分钟的模拟饮食侵蚀挑战。此外，对30个无薄膜的牙釉质样本也进行了该挑战。轮廓测定法显示，侵蚀受试者的样本平均台阶高度为1.74µm（标准差0.88），中位粗糙度（Sa）为0.39µm（四分位间距，IQR 0.3 - 0.56），与对照组平均台阶高度为1.34µm（标准差0.66）和0.33µm（IQR 0.27 - 0.38）相比，无统计学差异。无薄膜的对照样本Sa为0.44µm（IQR 0.36 - 0.69），与健康受试者的样本相比这些差异具有统计学意义（p = 0.002）。侵蚀组在100克负荷下的平均（标准差）显微硬度降低为113.5（10）KHN，健康受试者为93（15.4）KHN，无薄膜的牙釉质样本为139.6（21.8）KHN，所有组之间均有统计学差异。显微硬度和粗糙度数据表明，在这些研究条件下薄膜会影响侵蚀。
