Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg 2125, Gauteng, South Africa.
Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg 2125, Gauteng, South Africa.
Biosens Bioelectron. 2014 Apr 15;54:1-6. doi: 10.1016/j.bios.2013.10.019. Epub 2013 Oct 30.
A rapid dual channel lateral flow assay for the detection of Mycobacterium Tuberculosis antibodies (MTB 38 kDa monoclonal antibody) in human blood was developed. The MTB 6-14-38 kDa fusion antigen and anti-Protein A were used as the capture proteins for test and control lines respectively. Protein A labeled 40 nm gold nanoparticles were used as the detection conjugate. Whole blood and serum were spiked with MTB 38 kDa monoclonal antibody to make a positive sample model. The developed lateral flow was used to test MTB 38 kDa monoclonal antibody, and a detection limit of 5 ng/ml was used as a cut-off concentration of the analytes. The effect of the analyte concentration on the MTB lateral flow assay was studied using the variation of the intensity obtained from a ESE Quanti reader. There was a direct correlation between the analyte (MTB 38 kDa monoclonal antibody) concentration and the intensity of the test line. The intensity increased with an increase in the concentration of MTB 38 kDa monoclonal antibody, while in contrast, an increase in analyte concentration decreased the intensity of the control line.
开发了一种快速双通道侧向流动测定法，用于检测人血液中的结核分枝杆菌抗体（MTB 38 kDa 单克隆抗体）。MTB 6-14-38 kDa 融合抗原和抗蛋白 A 分别用作测试和控制线的捕获蛋白。蛋白 A 标记的 40nm 金纳米颗粒用作检测结合物。全血和血清中加入 MTB 38 kDa 单克隆抗体制成阳性样本模型。使用开发的侧向流动测定法检测 MTB 38 kDa 单克隆抗体，以 5ng/ml 作为分析物的截止浓度。使用 ESE Quanti 读取器获得的强度变化研究分析物浓度对 MTB 侧向流动测定法的影响。分析物（MTB 38 kDa 单克隆抗体）浓度与测试线的强度之间存在直接相关性。随着 MTB 38 kDa 单克隆抗体浓度的增加，强度增加，而相反，分析物浓度的增加会降低控制线的强度。
