Houlden B A, Hogarth P M, McKenzie I F
Immunogenetics. 1986;23(4):226-32. doi: 10.1007/BF00373017.
Competitive binding studies and immunoprecipitation experiments define at least five distinct epitopes encoded by Ly-6-linked genes--Ly-6A.2, Ly-6B.2, Ly-6C.2, Ly-6D.2, and ThB. Ly-6A.2, a 33 kd protein, and Ly-6D.2 are closely overlapping epitopes that can be distinguished by their unique thymus reactions of 10-20% or greater than 90%, respectively. Similarly, the Ly-6C.2 antigen present on a 14 kd moiety loosely overlaps the Ly-6B.2 antigen. Ly-6C.2 and Ly-6B.2 antigens are distinct from Ly-6A.2 and Ly-6D.2, however. ThB is a 16-18 kd antigen which is not associated on the cell surface with any other "Ly-6" antigens. In addition, independently derived antibodies made to the Ly-6C.2 antigen detect an identical epitope, as do antibodies to Ly-6A.2 and Ly-6B.2. These results imply the existence of a single antigenic site on each of these molecules.
竞争性结合研究和免疫沉淀实验确定了至少五个由Ly-6连锁基因编码的不同表位——Ly-6A.2、Ly-6B.2、Ly-6C.2、Ly-6D.2和ThB。Ly-6A.2是一种33kd的蛋白质，Ly-6D.2与之表位紧密重叠，但可分别通过其独特的胸腺反应（分别为10%-20%或大于90%）来区分。同样，存在于14kd部分的Ly-6C.2抗原与Ly-6B.2抗原表位松散重叠。然而，Ly-6C.2和Ly-6B.2抗原与Ly-6A.2和Ly-6D.2不同。ThB是一种16-18kd的抗原，在细胞表面不与任何其他“Ly-6”抗原相关联。此外，针对Ly-6C.2抗原制备的独立衍生抗体检测到相同的表位，针对Ly-6A.2和Ly-6B.2的抗体也是如此。这些结果表明这些分子中的每一个都存在单个抗原位点。
