Auchincloss H, Ozato K, Sachs D H
J Immunol. 1981 Nov;127(5):1839-43.
Two separate monoclonal antibodies, 34-11-3S and 34-2-11S, produced by the fusion of C3H anti-BDF1 sensitized spleen cells to the SP2/0 myeloma variant cell line, were found to detect alloantigens on subpopulations of murine lymphocytes. Analysis of the strain specificities of these antibodies showed that both detected antigens determined by genes closely linked to the Ly-6 locus. However, analysis of the tissue distributions of antigen-bearing cells indicated that the 2 antibodies reacted with distinct antigens, only 1 of which was similar in distribution to the classically described Ly-6.2 antigen. Functional studies performed using cells treated with antibody plus C showed that these 2 antibodies defined lymphocyte subpopulations that are different from each other and from those defined by several other reported Ly-6-related antibodies. The data suggest that the Ly-6 locus consists either of multiple tightly linked genes exhibiting marked linkage disequilibrium or of a single gene, the protein product of which is subject to post-translational modifications during cell differentiation leading to the expression of new determinants.
通过将C3H抗BDF1致敏的脾细胞与SP2/0骨髓瘤变异细胞系融合产生的两种独立的单克隆抗体34-11-3S和34-2-11S，被发现可检测鼠淋巴细胞亚群上的同种抗原。对这些抗体的品系特异性分析表明，两者均检测到由与Ly-6基因座紧密连锁的基因所决定的抗原。然而，对抗抗原细胞的组织分布分析表明，这两种抗体与不同的抗原发生反应，其中只有一种在分布上与经典描述的Ly-6.2抗原相似。使用抗体加C处理的细胞进行的功能研究表明，这两种抗体定义的淋巴细胞亚群彼此不同，也与其他几种报道的Ly-6相关抗体所定义的亚群不同。数据表明，Ly-6基因座要么由多个表现出明显连锁不平衡的紧密连锁基因组成，要么由单个基因组成，其蛋白质产物在细胞分化过程中会经历翻译后修饰，从而导致新决定簇的表达。
