Takei F
Immunogenetics. 1982;16(3):201-8. doi: 10.1007/BF00343309.
H9/25, an allospecificity encoded by the Ly-6 region, was biochemically characterized. It was sensitive to pepsin and heat treatment, but was resistant to periodate oxidation. Its apparent molecular weight was approximately 12000 daltons by gel filtration. The antigenic molecule was partially purified by gel filtration and antibody affinity chromatography. The partially purified antigen molecule was radioiodinated, immunoprecipitated with monoclonal antibody H9/25, and analyzed by SDS-polyacrylamide gel electrophoresis. The autoradiograph showed the molecular weight of H9/25 to be approximately 15000 daltons under reducing conditions. These results indicate that H9/25 is a protein with a single polypeptide chain of 12000-15000 daltons molecular weight, and the antigenic specificity is carried by a peptide but not a carbohydrate moiety.
对由Ly-6区域编码的同种特异性H9/25进行了生化特性分析。它对胃蛋白酶和热处理敏感，但对高碘酸盐氧化具有抗性。通过凝胶过滤法测得其表观分子量约为12000道尔顿。抗原分子通过凝胶过滤和抗体亲和层析进行了部分纯化。将部分纯化的抗原分子进行放射性碘化，用单克隆抗体H9/25进行免疫沉淀，然后通过SDS-聚丙烯酰胺凝胶电泳进行分析。放射自显影片显示，在还原条件下H9/25的分子量约为15000道尔顿。这些结果表明，H9/25是一种分子量为12000 - 15000道尔顿的单条多肽链蛋白质，其抗原特异性由肽而非碳水化合物部分携带。
