滨海黎芦天然居群中质体 DNA 多样性表现出性表型和线粒体 DNA 的额外变异。

Plastid DNA diversity in natural populations of Beta maritima showing additional variation in sexual phenotype and mitochondrial DNA.

机构信息

Botanical Institute of the University of Düsseldorf, Universitätsstr. 1, 1, Düsseldorf, FRG.

出版信息

Theor Appl Genet. 1991 Apr;81(4):533-6. doi: 10.1007/BF00219445.

DOI:10.1007/BF00219445

Abstract

Plants of two natural populations of Beta maritima, characterized by high percentages of male-sterile plants, have been investigated for organelle DNA polymorphism. We confirm the two classes of mitochondrial DNA variation previously described: (i) mitochondrial DNA (mtDNA) type N is associated with male fertility, whereas mtDNA type S can cause cytoplasmic male sterility (CMS); (ii) the 10.4-kb linear plasmid is observed in both types of mitochondria and is not correlated with the cytoplasmic male sterility occurring in this plant material. A third polymorphism is now described for chloroplast DNA (ctDNA). This polymorphism occurs within single populations of Beta maritima. Three different ctDNA types have been identified by HindIII restriction analysis. Among the plants studied, ctDNA type 1 is associated with N mitochondria and type 2 with S mitochondria. Chloroplast DNA type 3 has been found both in a fertile N plant and in a sterile S plant. This finding suggests that the chloroplast DNA polymorphism reported is not involved in the expression of male sterility. A comparison with Beta vulgaris indicates that ctDNA type 3 of Beta maritima corresponds to the ctDNA of fertile sugar beet maintainer lines. The three types of Beta maritima ctDNA described in this study differ from the ctDNA of male-sterile sugar beet.

摘要

对来自两个自然居群的 Beta maritima 植物（其雄性不育植株的比例较高）的细胞器 DNA 多态性进行了研究。我们证实了先前描述的两类线粒体 DNA 变异：（i）线粒体 DNA（mtDNA）类型 N 与雄性可育有关，而 mtDNA 类型 S 可导致细胞质雄性不育（CMS）；（ii）10.4kb 线性质体在两种类型的线粒体中都存在，并且与该植物材料中发生的细胞质雄性不育无关。现在对叶绿体 DNA（ctDNA）也描述了第三种多态性。这种多态性发生在单个 Beta maritima 种群中。通过 HindIII 限制分析鉴定出三种不同的 ctDNA 类型。在所研究的植物中，ctDNA 类型 1 与 N 线粒体相关，类型 2 与 S 线粒体相关。在一个可育的 N 植物和一个不育的 S 植物中都发现了 ctDNA 类型 3。这一发现表明，报告的叶绿体 DNA 多态性与雄性不育的表达无关。与 Beta vulgaris 的比较表明，Beta maritima 的 ctDNA 类型 3 对应于可育糖甜菜保持系的 ctDNA。本研究中描述的三种 Beta maritima ctDNA 与雄性不育糖甜菜的 ctDNA 不同。