Department of Genetics, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel.
Theor Appl Genet. 1989 Aug;78(2):293-7. doi: 10.1007/BF00288814.
Mitochondrial DNA (mtDNA) from fertile (N) and possibly new cytoplasmic male sterile (CMS) genotypes was studied in the sugar beet Beta vulgaris L. It was found by restriction endonuclease analysis that BMC-CMS, a cytoplasm that was derived from the wild beet Beta maritima, contained a unique type of mtDNA which is distinguishable from both the N and S-CMS, the only other CMS genotype that is currently availabe in B. vulgaris L. The organization of three genes: coxI, coxII and cob, was analyzed by hybridization with heterologous probes from maize. These genes have a similar structure in N and BMC-CMS that is different from S-CMS. It is concluded that BMC-CMS is a novel CMS genotype in the sugar beet.
对来自可育(N)和可能的新型细胞质雄性不育(CMS)基因型的线粒体 DNA(mtDNA)进行了研究,研究对象是糖甜菜 Beta vulgaris L。通过限制内切酶分析发现,源自野生甜菜 Beta maritima 的 BMC-CMS 细胞质含有一种独特的 mtDNA 类型,与 N 和 S-CMS 不同,S-CMS 是目前在 B. vulgaris L 中唯一可用的 CMS 基因型。使用来自玉米的异源探针进行杂交,分析了三个基因:coxI、coxII 和 cob 的组织。这些基因在 N 和 BMC-CMS 中的结构与 S-CMS 不同。因此,可以得出结论,BMC-CMS 是糖甜菜中的一种新型 CMS 基因型。