Brown J W, Reeve J N
J Bacteriol. 1986 May;166(2):686-8. doi: 10.1128/jb.166.2.686-688.1986.
Polyadenylated [poly(A)+] RNA has been isolated from the halophilic archaebacterium Halobacterium halobium by binding, at 4 degrees C, to oligo(dT)-cellulose. H. halobium contains approximately 12 times more poly(A) per unit of RNA than does the methanogenic archaebacterium Methanococcus vannielii. The 3' poly(A) tracts in poly(A)+ RNA molecules are approximately twice as long (average length of 20 nucleotides) in H. halobium as in M. vannielii. In both archaebacterial species, poly(A)+ RNAs are unstable.
通过在4℃下与寡聚(dT)纤维素结合,从嗜盐古细菌盐生盐杆菌中分离出聚腺苷酸化[poly(A)+]RNA。盐生盐杆菌每单位RNA中含有的聚腺苷酸比产甲烷古细菌万氏甲烷球菌多约12倍。盐生盐杆菌中poly(A)+RNA分子中的3'聚腺苷酸尾大约是万氏甲烷球菌中的两倍长(平均长度为20个核苷酸)。在这两种古细菌中,poly(A)+RNA都是不稳定的。